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cmazzoni
Genome_Evaluation_Pipeline
Commits
7791c651
Commit
7791c651
authored
3 years ago
by
james94
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memory
parent
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configuration/define_resources.yaml
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configuration/define_resources.yaml
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configuration/define_resources.yaml
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92
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97
View file @
7791c651
...
@@ -10,25 +10,25 @@
...
@@ -10,25 +10,25 @@
unzipHiC_R1
:
unzipHiC_R1
:
jobname
:
GEP.{rule}.{wildcards.asmID}
jobname
:
GEP.{rule}.{wildcards.asmID}
mem
ory
:
120
00
mem
_mb
:
5
00
time
:
"
0
0
:30:05"
time
:
"
0
1
:30:05"
threads
:
8
threads
:
4
# symlinkUnzippedHiC_R1:
# symlinkUnzippedHiC_R1:
# jobname: GEP.{rule}.{wildcards.asmID}
# jobname: GEP.{rule}.{wildcards.asmID}
# mem
ory
: 10
# mem
_mb
: 10
# time: "00:05:00"
# time: "00:05:00"
# threads: 1
# threads: 1
unzipHiC_R2
:
unzipHiC_R2
:
jobname
:
GEP.{rule}.{wildcards.asmID}
jobname
:
GEP.{rule}.{wildcards.asmID}
mem
ory
:
120
00
mem
_mb
:
5
00
time
:
"
0
0
:30:05"
time
:
"
0
1
:30:05"
threads
:
8
threads
:
4
# symlinkUnzippedHiC_R2:
# symlinkUnzippedHiC_R2:
# jobname: GEP.{rule}.{wildcards.asmID}
# jobname: GEP.{rule}.{wildcards.asmID}
# mem
ory
: 10
# mem
_mb
: 10
# time: "00:05:00"
# time: "00:05:00"
# threads: 1
# threads: 1
...
@@ -36,98 +36,98 @@ unzipHiC_R2:
...
@@ -36,98 +36,98 @@ unzipHiC_R2:
pretext_index_PRI_asm
:
pretext_index_PRI_asm
:
jobname
:
GEP.{rule}.{wildcards.asmID}
jobname
:
GEP.{rule}.{wildcards.asmID}
mem
ory
:
72000
mem
_mb
:
72000
time
:
"
02:30:00"
time
:
"
02:30:00"
threads
:
2
threads
:
1
pretext_fastq2bam_R1
:
pretext_fastq2bam_R1
:
jobname
:
GEP.{rule}.{wildcards.asmID}
jobname
:
GEP.{rule}.{wildcards.asmID}
mem
ory
:
32000
mem
_mb
:
32000
time
:
"
08:00:00"
time
:
"
08:00:00"
threads
:
12
threads
:
12
pretext_fastq2bam_R2
:
pretext_fastq2bam_R2
:
jobname
:
GEP.{rule}.{wildcards.asmID}
jobname
:
GEP.{rule}.{wildcards.asmID}
mem
ory
:
32000
mem
_mb
:
32000
time
:
"
08:00:00"
time
:
"
08:00:00"
threads
:
12
threads
:
12
pretext_filter_5primeEnd_R1
:
pretext_filter_5primeEnd_R1
:
jobname
:
GEP.{rule}.{wildcards.asmID}
jobname
:
GEP.{rule}.{wildcards.asmID}
mem
ory
:
200
mem
_mb
:
200
time
:
"
08:00:00"
time
:
"
08:00:00"
threads
:
4
threads
:
4
pretext_filter_5primeEnd_R2
:
pretext_filter_5primeEnd_R2
:
jobname
:
GEP.{rule}.{wildcards.asmID}
jobname
:
GEP.{rule}.{wildcards.asmID}
mem
ory
:
200
mem
_mb
:
200
time
:
"
08:00:00"
time
:
"
08:00:00"
threads
:
4
threads
:
4
pretext_filtered_combine
:
pretext_filtered_combine
:
jobname
:
GEP.{rule}.{wildcards.asmID}
jobname
:
GEP.{rule}.{wildcards.asmID}
mem
ory
:
9500
mem
_mb
:
9500
time
:
"
08:30:00"
time
:
"
08:30:00"
threads
:
12
threads
:
12
pretext_map
:
pretext_map
:
jobname
:
GEP.{rule}.{wildcards.asmID}
jobname
:
GEP.{rule}.{wildcards.asmID}
mem
ory
:
32000
mem
_mb
:
32000
time
:
"
08:30:00"
time
:
"
08:30:00"
threads
:
12
threads
:
12
pretext_snapshot
:
pretext_snapshot
:
jobname
:
GEP.{rule}.{wildcards.asmID}
jobname
:
GEP.{rule}.{wildcards.asmID}
mem
ory
:
500
mem
_mb
:
500
time
:
"
00:10:00"
time
:
"
00:10:00"
threads
:
1
threads
:
1
pretextMaps2md
:
#
pretextMaps2md:
jobname
:
GEP.{rule}.{wildcards.asmID}
#
jobname: GEP.{rule}.{wildcards.asmID}
mem
ory
:
500
#
mem
_mb
: 500
time
:
"
00:10:00"
#
time: "00:10:00"
threads
:
1
#
threads: 1
unzipFasta_PRI
:
unzipFasta_PRI
:
jobname
:
GEP.{rule}.{wildcards.asmID}
jobname
:
GEP.{rule}.{wildcards.asmID}
mem
ory
:
500
mem
_mb
:
500
time
:
"
00:
0
5:00"
time
:
"
00:
1
5:00"
threads
:
4
threads
:
4
# symlinkUnzippedFasta_PRI:
# symlinkUnzippedFasta_PRI:
# jobname: GEP.{rule}.{wildcards.asmID}
# jobname: GEP.{rule}.{wildcards.asmID}
# mem
ory
: 500
# mem
_mb
: 500
# time: "00:
0
5:00"
# time: "00:
1
5:00"
# threads:
# threads:
1
unzipFasta_ALT
:
unzipFasta_ALT
:
jobname
:
GEP.{rule}.{wildcards.asmID}
jobname
:
GEP.{rule}.{wildcards.asmID}
memory
:
500
mem_mb
:
500
time
:
"
00:05:00"
time
:
"
00:15:00"
threads
:
14
threads
:
4
#
# symlinkUnzippedFasta_ALT:
# symlinkUnzippedFasta_ALT:
# jobname: GEP.{rule}.{wildcards.asmID}
# jobname: GEP.{rule}.{wildcards.asmID}
# mem
ory
: 500
# mem
_mb
: 500
# time: "00:05:05"
# time: "00:05:05"
# threads: 1
# threads: 1
merqury
:
merqury
:
jobname
:
GEP.{rule}.{wildcards.asmID}
jobname
:
GEP.{rule}.{wildcards.asmID}
mem
ory
:
40000
mem
_mb
:
40000
time
:
"
08:00:00"
time
:
"
08:00:00"
threads
:
12
threads
:
8
busco5
:
busco5
:
jobname
:
GEP.{rule}.{wildcards.asmID}
jobname
:
GEP.{rule}.{wildcards.asmID}
mem
ory
:
150000
mem
_mb
:
150000
time
:
"
1-00:00:00"
time
:
"
1-00:00:00"
threads
:
16
threads
:
16
...
@@ -135,7 +135,7 @@ busco5:
...
@@ -135,7 +135,7 @@ busco5:
# moveBuscoOutputs:
# moveBuscoOutputs:
# jobname: GEP.{rule}.{wildcards.asmID}
# jobname: GEP.{rule}.{wildcards.asmID}
# mem
ory
: 500
# mem
_mb
: 500
# time: "00:05:05"
# time: "00:05:05"
# threads: 1
# threads: 1
...
@@ -148,60 +148,60 @@ busco5:
...
@@ -148,60 +148,60 @@ busco5:
genomescope2
:
genomescope2
:
jobname
:
GEP.{rule}.{wildcards.asmID}.{wildcards.kmer}
jobname
:
GEP.{rule}.{wildcards.asmID}.{wildcards.kmer}
mem
ory
:
500
mem
_mb
:
500
time
:
"
00:
0
5:00"
time
:
"
00:
1
5:00"
threads
:
1
threads
:
1
assemblyStats
:
assemblyStats
:
jobname
:
GEP.{rule}.{wildcards.asmID}
jobname
:
GEP.{rule}.{wildcards.asmID}
mem
ory
:
500
mem
_mb
:
500
time
:
"
00:
0
5:00"
time
:
"
00:
1
5:00"
threads
:
1
threads
:
1
# saveConfiguration_and_getKeyValues_kmer:
# saveConfiguration_and_getKeyValues_kmer:
# jobname: GEP.{rule}.{wildcards.asmID}.{wildcards.kmer}
# jobname: GEP.{rule}.{wildcards.asmID}.{wildcards.kmer}
# mem
ory
: 500
# mem
_mb
: 500
# time: "00:05:00"
# time: "00:05:00"
# threads: 1
# threads: 1
#
#
#
#
# saveConfiguration_and_getKeyValues:
# saveConfiguration_and_getKeyValues:
# jobname: GEP.{rule}.{wildcards.asmID}
# jobname: GEP.{rule}.{wildcards.asmID}
# mem
ory
: 500
# mem
_mb
: 500
# time: "00:05:05"
# time: "00:05:05"
# threads: 1
# threads: 1
#
#
#
#
# aggregateAllAssemblies:
# aggregateAllAssemblies:
# jobname: GEP.{rule}
# jobname: GEP.{rule}
# mem
ory
: 500
# mem
_mb
: 500
# time: "00:05:05"
# time: "00:05:05"
# threads: 1
# threads: 1
#
# makeReport:
# makeReport:
# jobname: GEP.{rule}.{wildcards.asmID}.{wildcards.kmer}
# jobname: GEP.{rule}.{wildcards.asmID}.{wildcards.kmer}
# mem
ory
: 500
# mem
_mb
: 500
# time: "00:05:05"
# time: "00:05:05"
# threads: 1
# threads: 1
#
#
#
#
# addFullTable:
# addFullTable:
# jobname: GEP.{rule}
# jobname: GEP.{rule}
# mem
ory
: 500
# mem
_mb
: 500
# time: "00:05:05"
# time: "00:05:05"
# threads: 1
# threads: 1
#
#
# aggregateReport:
# aggregateReport:
# jobname: GEP.{rule}
# jobname: GEP.{rule}
# mem
ory
: 500
# mem
_mb
: 500
# time: "00:05:05"
# time: "00:05:05"
# threads: 1
# threads: 1
makePDF
:
makePDF
:
jobname
:
GEP.{rule}
jobname
:
GEP.{rule}
mem
ory
:
1000
mem
_mb
:
1000
time
:
"
00:10:05"
time
:
"
00:10:05"
threads
:
1
threads
:
1
...
@@ -209,26 +209,26 @@ makePDF:
...
@@ -209,26 +209,26 @@ makePDF:
unzipFastq_R1
:
unzipFastq_R1
:
jobname
:
GEP.{rule}.{wildcards.sample}.{wildcards.readCounter}.{wildcards.trim10x}.{wildcards.trimAdapters}
jobname
:
GEP.{rule}.{wildcards.sample}.{wildcards.readCounter}.{wildcards.trim10x}.{wildcards.trimAdapters}
mem
ory
:
96000
mem
_mb
:
96000
time
:
"
0
0:30
:05"
time
:
"
0
1:45
:05"
threads
:
2
threads
:
4
unzipFastq_R2
:
unzipFastq_R2
:
jobname
:
GEP.{rule}.{wildcards.sample}.{wildcards.readCounter}.{wildcards.trim10x}.{wildcards.trimAdapters}
jobname
:
GEP.{rule}.{wildcards.sample}.{wildcards.readCounter}.{wildcards.trim10x}.{wildcards.trimAdapters}
mem
ory
:
96000
mem
_mb
:
96000
time
:
"
0
0:30
:05"
time
:
"
0
1:45
:05"
threads
:
4
threads
:
4
# symlinkUnzippedFastq_R1:
# symlinkUnzippedFastq_R1:
# jobname: GEP.{rule}.{wildcards.sample}.{wildcards.readCounter}.{wildcards.trim10x}.{wildcards.trimAdapters}
# jobname: GEP.{rule}.{wildcards.sample}.{wildcards.readCounter}.{wildcards.trim10x}.{wildcards.trimAdapters}
# mem
ory
: 500
# mem
_mb
: 500
# time: "00:01:35"
# time: "00:01:35"
# threads: 1
# threads: 1
#
#
# symlinkUnzippedFastq_R2:
# symlinkUnzippedFastq_R2:
# jobname: GEP.{rule}.{wildcards.sample}.{wildcards.readCounter}.{wildcards.trim10x}.{wildcards.trimAdapters}
# jobname: GEP.{rule}.{wildcards.sample}.{wildcards.readCounter}.{wildcards.trim10x}.{wildcards.trimAdapters}
# mem
ory
: 500
# mem
_mb
: 500
# time: "00:01:35"
# time: "00:01:35"
# threads: 1
# threads: 1
...
@@ -237,75 +237,75 @@ unzipFastq_R2:
...
@@ -237,75 +237,75 @@ unzipFastq_R2:
# symLink_trim10xbarcodes_notrimAdapt:
# symLink_trim10xbarcodes_notrimAdapt:
# jobname: GEP.{rule}.{wildcards.sample}.{wildcards.readCounter}
# jobname: GEP.{rule}.{wildcards.sample}.{wildcards.readCounter}
# mem
ory
: 500
# mem
_mb
: 500
# time: "00:01:35"
# time: "00:01:35"
# threads: 1
# threads: 1
#
#
# symlinks_no10xOrAdaptTrim:
# symlinks_no10xOrAdaptTrim:
# jobname: GEP.{rule}.{wildcards.sample}.{wildcards.readCounter}
# jobname: GEP.{rule}.{wildcards.sample}.{wildcards.readCounter}
# mem
ory
: 500
# mem
_mb
: 500
# time: "00:01:35"
# time: "00:01:35"
# threads: 1
# threads: 1
#
#
#
#
# symlinks_no10xwithAdaptTrim:
# symlinks_no10xwithAdaptTrim:
# jobname: GEP.{rule}.{wildcards.sample}.{wildcards.readCounter}
# jobname: GEP.{rule}.{wildcards.sample}.{wildcards.readCounter}
# mem
ory
: 500
# mem
_mb
: 500
# time: "00:01:35"
# time: "00:01:35"
# threads: 1
# threads: 1
#
#
#
#
# symlink_trim10xbarcodesR2:
# symlink_trim10xbarcodesR2:
# jobname: GEP.{rule}.{wildcards.sample}.{wildcards.readCounter}.{wildcards.trimAdapters}
# jobname: GEP.{rule}.{wildcards.sample}.{wildcards.readCounter}.{wildcards.trimAdapters}
# mem
ory
: 500
# mem
_mb
: 500
# time: "00:01:35"
# time: "00:01:35"
# threads: 1
# threads: 1
trim10xbarcodes
:
trim10xbarcodes
:
jobname
:
GEP.{rule}.{wildcards.sample}.{wildcards.readCounter}.{wildcards.trimAdapters}
jobname
:
GEP.{rule}.{wildcards.sample}.{wildcards.readCounter}.{wildcards.trimAdapters}
mem
ory
:
500
mem
_mb
:
500
time
:
"
0
4:0
0:05"
time
:
"
0
2:3
0:05"
threads
:
4
threads
:
4
trimAdapters
:
trimAdapters
:
jobname
:
GEP.{rule}.{wildcards.sample}.{wildcards.readCounter}.{wildcards.trim10x}
jobname
:
GEP.{rule}.{wildcards.sample}.{wildcards.readCounter}.{wildcards.trim10x}
mem
ory
:
500
mem
_mb
:
500
time
:
"
0
4:0
0:05"
time
:
"
0
2:3
0:05"
threads
:
8
threads
:
8
fastqc_Illumina
:
fastqc_Illumina
:
jobname
:
GEP.{rule}.{wildcards.sample}.{wildcards.readCounter}.{wildcards.trimAdapters}.{wildcards.trim10x}
jobname
:
GEP.{rule}.{wildcards.sample}.{wildcards.readCounter}.{wildcards.trimAdapters}.{wildcards.trim10x}
mem
ory
:
1000
mem
_mb
:
1000
time
:
"
04:00:05"
time
:
"
04:00:05"
threads
:
4
threads
:
4
# multiqc_hifi:
# multiqc_hifi:
# jobname: GEP.{rule}.{wildcards.sample}.{wildcards.trimAdapters}.{wildcards.trim10x}
# jobname: GEP.{rule}.{wildcards.sample}.{wildcards.trimAdapters}.{wildcards.trim10x}
# mem
ory
: 500
# mem
_mb
: 500
# time: "01:00:05"
# time: "01:00:05"
# threads: 1
# threads: 1
meryl_R1
:
meryl_R1
:
jobname
:
GEP.{rule}.{wildcards.sample}.{wildcards.readCounter}.{wildcards.trimAdapters}.{wildcards.trim10x}.{wildcards.kmer}
jobname
:
GEP.{rule}.{wildcards.sample}.{wildcards.readCounter}.{wildcards.trimAdapters}.{wildcards.trim10x}.{wildcards.kmer}
mem
ory
:
20000
mem
_mb
:
20000
time
:
"
01:
0
0:05"
time
:
"
01:
3
0:05"
threads
:
8
threads
:
12
meryl_R2
:
meryl_R2
:
jobname
:
GEP.{rule}.{wildcards.sample}.{wildcards.readCounter}.{wildcards.trimAdapters}.{wildcards.trim10x}.{wildcards.kmer}
jobname
:
GEP.{rule}.{wildcards.sample}.{wildcards.readCounter}.{wildcards.trimAdapters}.{wildcards.trim10x}.{wildcards.kmer}
mem
ory
:
20000
mem
_mb
:
20000
time
:
"
01:
0
0:05"
time
:
"
01:
3
0:05"
threads
:
8
threads
:
12
meryl_illumina_build
:
meryl_illumina_build
:
jobname
:
GEP.{rule}.{wildcards.sample}.{wildcards.trimAdapters}.{wildcards.trim10x}.{wildcards.kmer}
jobname
:
GEP.{rule}.{wildcards.sample}.{wildcards.trimAdapters}.{wildcards.trim10x}.{wildcards.kmer}
mem
ory
:
30000
mem
_mb
:
30000
time
:
"
0
0
:45:05"
time
:
"
0
2
:45:05"
threads
:
8
threads
:
12
...
@@ -313,48 +313,43 @@ meryl_illumina_build:
...
@@ -313,48 +313,43 @@ meryl_illumina_build:
###### HIFI BUILD #######
###### HIFI BUILD #######
unzipHifi
:
unzipHifi
:
#
jobname: GEP.{rule}.{wildcards.sample}.{wildcards.readCounter}.{wildcards.smrtornot}
jobname
:
GEP.{rule}.{wildcards.sample}.{wildcards.readCounter}.{wildcards.smrtornot}
mem
ory
:
128000
mem
_mb
:
128000
time
:
"
00:30:05"
time
:
"
00:30:05"
threads
:
4
# symlinkUnzippedHifi:
# symlinkUnzippedHifi:
# jobname: GEP.{rule}.{wildcards.sample}.{wildcards.readCounter}.{wildcards.smrtornot}
# jobname: GEP.{rule}.{wildcards.sample}.{wildcards.readCounter}.{wildcards.smrtornot}
# mem
ory
: 500
# mem
_mb
: 500
# time: "00:05:05"
# time: "00:05:05"
# symlinkfornotSmartTrimmed:
# symlinkfornotSmartTrimmed:
# jobname: GEP.{rule}.{wildcards.sample}.{wildcards.readCounter}
# jobname: GEP.{rule}.{wildcards.sample}.{wildcards.readCounter}
# mem
ory
: 500
# mem
_mb
: 500
# time: "00:05:05"
# time: "00:05:05"
fastqc_hifi
:
fastqc_hifi
:
#
jobname: GEP.{rule}.{wildcards.sample}.{wildcards.readCounter}.{wildcards.smrtornot}
jobname
:
GEP.{rule}.{wildcards.sample}.{wildcards.readCounter}.{wildcards.smrtornot}
mem
ory
:
12000
mem
_mb
:
12000
time
:
"
04:00:05"
time
:
"
04:00:05"
threads
:
4
#
multiqc_hifi:
multiqc_hifi
:
#
#
jobname: GEP.{rule}.{wildcards.sample}.{wildcards.readCounter}.{wildcards.smrtornot}
jobname
:
GEP.{rule}.{wildcards.sample}.{wildcards.readCounter}.{wildcards.smrtornot}
#
mem
ory
: 4000
mem
_mb
:
4000
#
time: "01:15:05"
time
:
"
01:15:05"
meryl_hifi_count
:
meryl_hifi_count
:
# jobname: GEP.{rule}.{wildcards.sample}.{wildcards.readCounter}.{wildcards.smrtornot}.{wildcards.kmer}
jobname
:
GEP.{rule}.{wildcards.sample}.{wildcards.readCounter}.{wildcards.smrtornot}.{wildcards.kmer}
memory
:
96000
mem_mb
:
96000
time
:
"
04:30:05"
time
:
"
02:30:05"
threads
:
16
meryl_hifi_build
:
meryl_hifi_build
:
# jobname: GEP.{rule}.{wildcards.sample}.{wildcards.smrtornot}.{wildcards.kmer}
jobname
:
GEP.{rule}.{wildcards.sample}.{wildcards.smrtornot}.{wildcards.kmer}
memory
:
96000
mem_mb
:
96000
time
:
"
04:30:05"
time
:
"
01:30:05"
threads
:
16
trimSMRTbell
:
trimSMRTbell
:
jobname
:
GEP.{rule}.{wildcards.sample}.{wildcards.readCounter}
jobname
:
GEP.{rule}.{wildcards.sample}.{wildcards.readCounter}
memory
:
96000
mem_mb
:
96000
time
:
"
04:30:05"
time
:
"
01:30:05"
threads
:
8
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